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Rats frequently form strong friendships with particular packmates, and can become depressed if separated from them. Wherever possible, avoid splitting up rats who are getting on well.
By the same token, they sometimes develop strong animosities, especially between bucks, and it may become necessary to separate cagemates who really seem to loathe each other.
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Bucks and does can nearly always be introduced together (if not then the aggressor is usually the doe), but will of course have kits unless they are sterile due to age or other cause (see reproduction). You can also introduce does of all ages together. It is rarely possible to introduce strange adult (i.e. much over 3 months) bucks together unless they are very old, but kitten bucks can be put with each other or, usually, with adult bucks. At a rough estimate I would say introducing young adult bucks to young adult bucks is only successful about 20% of the time, whereas the success-rate of other combinations is about 90%.
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When you first introduce rats on neutral territory they are usually perfectly OK together, and may even ignore each other. Often it is only after a few hours that they begin to sniff and possibly fight each other. It seems they are prepared to ignore strangers who they think are "just passing through", but once they realize they are sticking around then the stranger looks like a challenger/invader and they have to sort out issues of territory and dominance. Very, very rarely a tiny minority of does can become really dangerous at this stage: see section on aggression.
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It is usually better to put two kittens in with adults, rather than one - two kits play together, but one on its own will try to play with the adults, and annoy them.
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New additions will be bullied and chased a bit at first, but this should only go on for a couple of days. If a resident animal seriously attacks a new one you may have to split them up.
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Norway rats have scent-glands along their flanks. When fighting, bucks often rub their sides against each other to transfer their scent: they will also rub scent all over their cages and other objects, leaving a pale brown greasy smear. Some very possessive bucks also urinate on items they wish to own - including you.
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When Norway rats fight they blow up their fur, hump their backs and prance sideways at each other, making themselves look as big as possible. They then scratch at each other with a back foot and and try to rub the scent-glands on their sides against each other. They also often get into a clinch where they stand up on their hind-legs and waltz nose to nose, holding each other off with their hands.
Eventually somebody's nerve breaks - and then they get chased and lightly beaten-up.
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A whistling squeal, like a thin high scream, is generally a sign of aggression rather than nervousness. It's equivalent to tomcats singing at each other.
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